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13817140470更新时间:2020-12-30 浏览次数:1095
【文献标题】Effects of glyphosate-based herbicide-contaminated diets on reproductive organ
toxicity and hypothalamic-pituitary-ovarian axis hormones in weaned piglets
【作者】Huiyang Fu, Feng Gao, Xiaoxu Wang, et.al
【作者单位】东北农业大学( Northeast Agricultural University)【文献中引用产品】
猪总抗体氧化能力(T-AOC)ELISA试剂盒
猪超氧化物歧化酶(SOD)ELISA试剂盒
猪谷胱甘肽过氧化物酶(GPX)ELISA试剂盒
猪过氧化氢酶(CAT)ELISA试剂盒
猪谷胱甘肽S转移酶(GST)ELISA试剂盒
猪还原型谷胱甘肽(GSH)ELISA试剂盒
猪丙二醛(MDA)ELISA试剂盒
猪过氧化氢(H2O2)ELISA试剂盒
【关键词】 glyphosate-based herbicide, weaned piglets, uterus, ovary, hypothalamic-pituitary-ovarian
【DOI】doi.org/10.1016/j.envpol.2020.115596
【影响因子(IF)】6.7
【出版期刊】《Environmental Pollution》
【产品原文引用】
Antioxidant level of uterus
A 1 g uterine sample was taken from -20 °C and mixed with 9 ml of phosphate-buffered saline (PBS, 0.01 mol L-1 170 , PH = 7.2-7.4). After 5 min of homogenization with a 5000-rpm high-speed homogenizer, and 10% tissue homogenate was prepared. This was centrifugated at 3500 rpm for 10 min, and the supernatant was taken for the detection of the antioxidant index. Kits for measuring the total protein(Code: JA10245), total antioxidant capacity (T-AOC) (Code: J-13106), superoxide dismutase (SOD) (Code: J-50065), glutathione peroxidase (GPx) (Code: J-53063), catalase (CAT) (Code: J-52619), glutathione S-transferase (GST) (Code: J-52618) enzyme activity and reduced glutathione (GSH) (Code:J-53087), malondialdehyde (MDA) (Code: J-58063), and hydrogen peroxide (H2O2) (Code: J-35958) content in tissues were purchased from Shanghai Jinma Laboratory Equipment Co., Ltd. (Jinma, Shanghai, China). All steps were performed in strict accordance with the kits’ instructions. The measured concentrations were based on the total protein mass of the uterus.
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