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【文献标题】Dynamic immune response characteristics of piglets infected with Actinobacillus pleuropneumoniae through omic

【作者】Rining Zhu, Hexiang Jianget.al

【作者单位】吉林大学(Jilin University)

【文献中引用产品】

猪铜锌超氧化物歧化酶(SOD1)ELISA试剂盒

猪微管蛋白α3A(TUBa4A)ELISA试剂盒

猪真核翻译起始因子4A1(eIF4A1)ELISA试剂盒

【关键词】Actinobacillus pleuropneumoniae, Porcine pleuropneumonia, Omics, Immune response

【DOI】doi.org/10.1186/s13568-021-01336-z

【出版期刊】《 AMB Express》

【产品原文引用】For the ELISA detection of TUBA4A, SOD1 and EIF4A in serum and BALF, we only analyzed samples from 12 to 120h post infection due to the shortage of the same batch of samples for omics detection. We found that the level of TUBA4A, SOD1 and EIF4A in BALF were consistent with the results of omics analysis (12 h/0 and 120 h/12 h) (Fig. 5A,B). Te level trends of TUBA4A, SOD1 and EIF4A in serum were also consistent with the omics data only at 12h, no diference or increase trend at 120h/12h in serum by ELISA assay. It was not similar with that in serum at 120h/24h, which might be due to the diferent sampling time points for ELISA (12?h) and for the omics analysis (24h), that is to say, these proteins levels may still be rising at 12?h, higher at 24h, so 120/24h decreased than 120h/12?h (Fig.7).

【文献标题】iTRAQ-based quantitative proteomic analysis of peripheral blood serum inpiglets infected with Actinobacillus pleuropneumoniae

【作者】Rining Zhu, Chuntong Baoet.al

【作者单位】吉林大学（ Jilin University）

【文献中引用产品】

猪甘露糖受体C1样蛋白1(MRC1)ELISA试剂盒

猪过氧化物酶2（PRDX2）ELISA试剂盒

猪补体因子B(CFB)ELISA

猪整合素β（ITG-β）ELISA试剂盒

猪E钙粘附分子(E-Cadherin)ELISA试剂盒

【关键词】A. pleuropneumoniae,iTRAQ,Porcine pleuropneumonia, Comparative proteomics, Serum

【DOI】doi.org/10.1186/s13568-020-01057-9

【出版期刊】《AMB Expr》

【产品原文引用】Five proteins were selected for validation by ELISA:

MRC1,PRDX2,CFB,ITGB and E-Cadherin. Te serum samples were allowed to clot for 1h at room temperature and then separated by centrifugation at 2000×g for 15min at 4°C. Te serum was then frozen until analyzed by ELISA. Te concentration of MRC1,PRDX2,CFB,ITGB and E-Cadherin was determined using commercial porcine ELISA kits (Shanghai Jinma Biological Technology, Inc., China) according to the manufacturer’s instructions. All samples were analyzed in duplicate. Cytokine concentrations were calculated using linear regression analysis based on the OD of the cytokine standards provided by the manufacturer.

【文献标题】Differences in pig respiratory tract and peripheral blood immune responses to Actinobacillus pleuropneumoniae

【作者】Chuntong Bao，Hexiang Jiang，Rining Zhu，et.al

【作者单位】吉林大学（Jilin University）

【文献中引用产品】

猪白细胞介素1α（IL-1α ）ELISA试剂盒

猪白细胞介素1β（IL-1β）ELISA试剂盒

猪白细胞介素1受体拮抗剂（IL-1Ra）ELISA试剂盒

猪白细胞介素2（IL-2）ELISA试剂盒

猪白细胞介素3（IL-3）ELISA试剂盒

猪白细胞介素4（IL-4）ELISA试剂盒

猪白细胞介素5（IL-5）ELISA试剂盒

猪白细胞介素6（IL-6）ELISA试剂盒

猪白细胞介素8（IL-8）ELISA试剂盒

猪白细胞介素2（IL-10）ELISA试剂盒

猪白细胞介素12（IL-12）ELISA试剂盒

猪白细胞介素13（IL-13）ELISA试剂盒

猪白细胞介素15（IL-15）ELISA试剂盒

猪白细胞介素-17A（IL-17A） ELISA试剂盒

猪白细胞介素18（IL-18）ELISA试剂盒

猪白细胞介素21（IL-21）ELISA试剂盒

猪白细胞介素22（IL-22）ELISA试剂盒

猪白细胞介素23（IL-23）ELISA试剂盒

猪白细胞介素32（IL-32）ELISA试剂盒

猪肿瘤坏死因子α（TNF-α）ELISA试剂盒

猪单核细胞趋化蛋白1（MCP-1/CCL2/MCAF）ELISA试剂盒

猪巨噬细胞炎性蛋白1β（MIP-1β/CCL4）ELISA试剂盒

猪正常T细胞表达和分泌因子（RANTES/CCL5）ELISA试剂盒

猪粒细胞巨噬细胞集落刺激因子（GM-CSF）ELISA试剂盒

猪α干扰素（IFN-α）ELISA试剂盒

猪干扰素诱导蛋白10（IP-10）ELISA试剂盒

猪转化生长因子β（TGF-β）ELISA试剂盒

猪CXC趋化因子配体9（CXCL9）ELISA试剂盒

猪趋化因子CCL3样蛋白1（CCL3L1）ELISA试剂盒

【DOI】doi.org/10.1016/j.vetmic.2020.108755

【出版期刊】《Veterinary Microbiology》

【产品原文引用】

7.Cytokine measurements Blood was collected from animals at 0/6/12/24/48/120 h post infection (n=3 each time point),incubated at 4°C for 30 min, and sera obtained after centrifugation. The sera and BALF from the same group were mixed together in equal proportion. Clear non-hemolyzed sera and BALF were stored at -80°C prior to biological analyses. The levels of IL-1α, IL-1β, IL-1Ra, IL-2, IL-3, IL-4,IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-15, IL-17A, IL-18, IL-21, IL-22, IL-23, IL-32, TNF-α,CCL2(MCP-1), CCL4 (MIP-1β), CCL5 (RANTES), GM-CSF, IFN-α, IP-10, TGF-β, CXCL9 and CCL3L1 were determined by ELISA according to the manufacturer’s protocols (Shanghai Jinma Biotechnology Co. Ltd.). The mixed sample had three technical replicates. A sample diluent was used to dilute the mixed sera and BALF samples five times according to the kit instructions, no other dilution was investigated.

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