溶壁酶/细胞溶解酶/Lyticase/Lysing Enzymes

 Description
Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product.
Application Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Biochem/physiol Actions Lyticase hydrolyzes poly-β（1→3）-glucose such as yeast cell wall glucan.
Other Notes View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Unit Definition One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Physical form Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers

Properties
form lyophilized powder
composition Protein, ≥20% biuret 
EQP level Premium
storage temp. −20°C

Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
Hazard Codes Xn
Risk Statements 42
Safety Statements 22
WGK Germany 3
 

• M0180磷酸烯醇式丙酮酸羧化酶/PEPC
• M0179葡萄糖脱氢酶/Glucose Dehydrogenase
• M0177单宁酶/鞣酸酶/丹宁酶/单宁酯酰水解酶/Tannase
• M0176植酸酶/肌醇六磷酸酶/Phytase from wheat
• M0174肠激酶/肠胨酶/肠蛋白酶/肠肽酶/肠激活酶/Enteropeptidase
• M0175亮氨酸氨基肽酶/亮氨酸氨肽酶/肽链外切酶/AP-M/LAP
• ACP酸性磷酸酶
• M0172α-甘油磷酸脱氢酶-磷酸丙糖异构酶/3-磷酸甘油脱氢酶-磷酸丙糖异构酶/GDH-TIM/GDH-TP
• M0171醛缩酶/丁醛醇酶/醇醛缩合酶/二磷酸果糖酶/丁酸醇酶/Aldolase from rabbit mu
• M0170蔗糖磷酸化酶/SP/Sucrose Phosphorylase