半乳糖氧化酶/Galactose oxidase

 Description
Application Useful in the determination of lactose.
Biochem/physiol Actions 2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.
Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.
Preparation Note Chromatographically purified
Unit Definition One unit will produce a ΔA425 of 1.0 per min at pH 6.0 at 25 °C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.
Physical form Lyophilized, contains buffer salts and stabilizer

Properties
composition Protein, 5-20% biuret 
storage temp. −20°C

Safety
WGK Germany 3

References
reference Avigad, G., et al. J. Biol. Chem. 237, 2736, （1962）

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