4,6-二脒基-2-苯基吲哚二盐酸盐/DAPI染色液

 Description
Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product.
Application DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Biochem/physiol Actions Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA （preferentially to AT rich DNA）, forming a stable complex which fluoresces approximay 20 times greater than DAPI alone.
Caution Protect from light.

Properties
assay ≥98% （HPLC and TLC）
form powder
fluorescence λex 340 nm; λem 488 nm （nur DAPI）
  λex 364 nm; λem 454 nm （DAPI-DNA-Komplex）
EQP level Premium
solubility H2O: soluble20 mg/mL （heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.）
  PBS: insoluble
abs. ε1mM/263 nm, H2O 30
storage temp. 2-8°C

Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 （US）, type P1 （EN143） respirator filter
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
WGK Germany 3

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